Concomitant Determination of Folar Nitrogen Loss, Net Carbon Dioxide Uptake, and Transpiration 1

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A closed system was designed for concomitant determination of net CO2 uptake, transpiration, and foliar nitrogen (N) loss in soybeans (Glycine max [L.] Merr.). The CO2 uptake was monitored by infrared analysis of system air removed periodically in aliquots. Leaf vapors were trapped in calibrated tubes at Dry Ice temperature, and transpiration rate was determined from the quantity of condensate. Subsequent pyrochemiluminescent analysis of this condensate revealed reduced N forms, although a [ill] percentage (4 to 15%) of oxidized forms was found.

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