Concomitant suppression of three target genes can explain the impact of a microRNA on metastasis
AUTOR(ES)
Valastyan, Scott
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
It remains unclear whether a microRNA (miRNA) affects a given phenotype via concomitant down-regulation of its entire repertoire of targets or instead by suppression of only a modest subset of effectors. We demonstrate that inhibition of breast cancer metastasis by miR-31—a miRNA predicted to modulate >200 mRNAs—can be entirely explained by miR-31's pleiotropic regulation of three targets. Thus, concurrent re-expression of integrin-α5, radixin, and RhoA abrogates miR-31-imposed metastasis suppression. These effectors influence distinct steps of the metastatic process. Our findings have implications concerning the importance of pleiotropy for the biological actions of miRNAs and provide mechanistic insights into metastasis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2779763Documentos Relacionados
- Specificity of microRNA target selection in translational repression
- Computational identification of Drosophila microRNA genes
- HIV-1 nef suppression by virally encoded microRNA
- Regulation of Flowering Time and Floral Organ Identity by a MicroRNA and Its APETALA2-Like Target Genes
- MicroRNA genes are transcribed by RNA polymerase II
