Conditions for Using DNA Polymerase I as an RNA-Dependent DNA Polymerase
AUTOR(ES)
Gulati, S. C.
RESUMO
Conditions are described for using Escherichia coli DNA polymerase I for synthesizing complementary DNA copies of natural RNA molecules, which are suitable for use in hybridization experiments. The molar ratio of enzyme to template is critical; below a certain level, synthesis is not observed. Hybrids formed with the complementary DNA are of comparable specificity and stability to those formed with complementary DNAs synthesized by viral RNA-directed DNA polymerase. Synthesis of dA-dT polymers, a common occurrence with this enzyme, can be eliminated by including distamycin in the reaction mixture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388156Documentos Relacionados
- An Oligonucleotide Affinity Column for RNA-Dependent DNA Polymerase from RNA Tumor Viruses
- Reticulocyte RNA-Dependent RNA Polymerase
- Mechanism of Carcinogenesis by RNA Tumor Viruses, I. An RNA-Dependent DNA Polymerase in Murine Sarcoma Viruses*
- Mechanixm of Carcinogenesis By RNA Tumor Viruses, I. An RNA-Dependent DNA Polymerase In Murine Sarcoma Viruses
- RNA-dependent RNA polymerase of tobacco plants
