Conformational transition in DNA on a cold surface
AUTOR(ES)
Feng, X. Z.
FONTE
Oxford University Press
RESUMO
The contour length of DNA fragments, deposited and imaged on mica under buffer, was measured as a function of deposition temperature. Extended DNA molecules (on Ni- and silane-treated surfaces) contract rapidly with falling temperature, approaching the contour length of A-DNA at 2°C. The contraction is not unique to a specific sequence and does not occur in solution at 2°C or on a surface at 25°C, indicating that it arises from a combination of low temperature and surface contact. It is probably a consequence of reduced water activity at a cold surface.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=102510Documentos Relacionados
- Mapping the B-A conformational transition along plasmid DNA
- Conformational Transition of Giant DNA in a Confined Space Surrounded by a Phospholipid Membrane
- Conformational DNA transition in the in vitro torsionally strained chicken beta-globin 5' region.
- Stereoelectronic effects on the transition barrier of polyproline conformational interconversion
- Conformational Transition in Oligopeptides: An NMR Spectroscopic Study