Conjugational Complementation Analysis of Transfer-Deficient Mutants of Flac in Escherichia coli
AUTOR(ES)
Achtman, Mark
RESUMO
A series of 102 transfer-deficient (tra−) mutants of Flac (84 of which have been previously described) were classified as carrying frameshift, amber, ochre, UGA, and nonsuppressible mutations. Techniques were evolved for using cultures of F prime strains as efficient recipients in matings, for measuring the number of (F′/F′) heterozygote cells in transient populations, and for measuring complementation between different Flac tra− mutants. These techniques were used to define nine tra cistrons and to assign most of the tra− mutations to one or other of these.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=247500Documentos Relacionados
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