Conserved genes encode guide RNAs in mitochondria of Crithidia fasciculata.
AUTOR(ES)
van der Spek, H
RESUMO
RNA editing is the post-transcriptional alteration of the nucleotide sequence of RNA, which in trypanosome mitochondria is characterized by the insertion and deletion of uridine residues. It has recently been proposed that the information for the sequence alteration in Leishmania tarentolae is provided by small guide (g) RNAs encoded in the mitochondrial DNA [Blum et al. (1990) Cell, 60, 189-198]. We are studying the mechanism of RNA editing in the insect trypanosome Crithidia fasciculata and report that: (i) a full length, conventional DNA gene or an independently replicating RNA gene that could encode the edited MURF3 transcript is absent when probed for in sensitive, calibrated assay systems; (ii) in all cases (seven) investigated in C. fasciculata so far, putative gRNA genes are found in a position in the mitochondrial DNA virtually identical to that in L. tarentolae and (iii) also in C. fasciculata, the putative gRNA genes are transcribed into small RNAs with discrete 5' ends. These results provide strong evolutionary evidence in support of the participation of gRNAs in RNA editing. Remarkably, in C. fasciculata the basepaired region of some putative gRNA:mRNA hybrids contains a C:A non-Watson-Crick basepair.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=452776Documentos Relacionados
- The nucleotide sequence of mitochondrial maxicircle genes of Crithidia fasciculata.
- Implications of novel guide RNA features for the mechanism of RNA editing in Crithidia fasciculata.
- Tubulin heterogeneity in the trypanosome Crithidia fasciculata.
- Pathway of protein glycosylation in the trypanosomatid Crithidia fasciculata.
- RNA editing in transcripts of the mitochondrial genes of the insect trypanosome Crithidia fasciculata.