Constancy in Human Sperm DNA Content
AUTOR(ES)
Sarkar, Siddhartha
RESUMO
The frequency distribution of DNA content of human sperm was measured in an automated flow microfluorometer. The flow method measures the DNA content by quantifying the amount of fluorescence emitted by the fluorescent Feulgen stained DNA of single sperm cells suspended in microdroplets. The variability in the mean value for the haploid amount of DNA in sperm from 15 randomly chosen donors was less than 1%. Statistical tests on the observed frequency distribution data indicated that each sperm population probably consists of two homogenous components present in almost equal proportions but differing in mean DNA content. The difference in their modal values for DNA is within the range of known values of DNA difference between the two sex chromosomes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433804Documentos Relacionados
- Population heterogeneity in human sperm DNA content.
- Methylation of DNA in early development: 5-methyl cytosine content of DNA in sea urchin sperm and embryos
- The Relative Constancy of the X-Ray-Induced Mutation Frequency of Drosophila Melanogaster Sperm in Inseminated Females
- Ascorbic acid protects against endogenous oxidative DNA damage in human sperm.
- Ribonuclease-sensitive DNA-synthesizing complex in human sperm heads and seminal fluid.