Constitutive and inducible kappa B binding activities in the cytosol of v-Rel-transformed lymphoid cells.
AUTOR(ES)
Hodgson, J
RESUMO
Constitutive and inducible kapp B binding activities associated with v-Rel and c-Rel in the cytosol of v-Rel-transformed cells have been identified. These activities were resolved by electrophoretic mobility shift assay and chromatographic techniques into a high-molecular-weight protein-DNA complex designated complex I containing v- and c-Rel and lower-molecular-weight complexes II, III and IV which contained only v-Rel and which were stimulated by nucleotides, low pH, and detergent. These experiments suggest that interaction of v-Rel and c-Rel decreases the DNA-binding activity of each.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=188819Documentos Relacionados
- Mutations in the DNA-binding and dimerization domains of v-Rel are responsible for altered kappa B DNA-binding complexes in transformed cells.
- Localization of the v-rel protein in reticuloendotheliosis virus strain T-transformed lymphoid cells.
- Two distinct mechanisms contribute to the constitutive activation of RelB in lymphoid cells.
- The v-rel oncogene product is complexed to a 40-kDa phosphoprotein in transformed lymphoid cells.
- NF-kappa B p100 is one of the high-molecular-weight proteins complexed with the v-Rel oncoprotein in transformed chicken spleen cells.