Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)
AUTOR(ES)
Xu, M
RESUMO
A mutant, Vp-MX02, having a deletion in the gene (trh) for the thermostable direct hemolysin-related hemolysin (TRH) was constructed by double-crossover gene conversion from a TRH-producing Vibrio parahaemolyticus strain, TH3996. The deleted region was the upstream half of trh. Hemolysis was completely lost, and TRH antigen was undetectable in the mutant. Administration of the mutant into ligated rabbit small intestines elicited partial, but apparent, fluid accumulation. These results suggest that the enterotoxicity of TRH-producing V. parahaemolyticus TH3996 may be attributed to the remaining C-terminal of TRH or, more likely, to an unknown virulence factor(s) in addition to TRH.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=196300Documentos Relacionados
- Construction and Characterization of an Isogenic Mutant of Vibrio parahaemolyticus Having a Deletion in the Thermostable Direct Hemolysin-Related Hemolysin Gene (trh)
- Sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of Vibrio parahaemolyticus.
- Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus.
- Analysis of the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes in urease-positive strains of Vibrio parahaemolyticus isolated on the West Coast of the United States.
- Purification and Characterization of Thermostable Direct Hemolysin of Vibrio parahaemolyticus