Construction of an adenovirus type 7a E1A- vector.
AUTOR(ES)
Abrahamsen, K
RESUMO
A strategy for constructing replication-defective adenovirus vectors from non-subgroup C viruses has been successfully demonstrated with adenovirus type 7 strain a (Ad7a) as the prototype. An E1A-deleted Ad7a reporter virus expressing the chloramphenicol acetyltransferase (CAT) gene from the cytomegalovirus promoter enhancer was constructed with DNA fragments isolated from Ad7a, an Ad7a recombination reporter plasmid, and the 293 cell line. The Ad7a-CAT virus particle transduces A549 cells as efficiently as Ad5-based vectors. Intravenous infections in a murine model indicate that the Ad7a-CAT virus infects a variety of tissues, with maximal levels of CAT gene expression found in the liver. The duration of Ad7a-CAT transgene expression in the liver was maximally maintained 2 weeks postinfection, with a decline to baseline activity by the week 4 postinfection. Ad7a-CAT represents the first example of a non-subgroup C E1A- adenovirus gene transfer vector.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=192370Documentos Relacionados
- Cell-type-specific synthesis of murine immunoglobulin mu RNA from an adenovirus vector.
- Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
- Construction of a regulated PGK expression vector.
- Correction of a deletion mutant by gene targeting with an adenovirus vector.
- Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.