Content of Adenosine Phosphates and Adenylate Energy Charge in Germinating Ponderosa Pine Seeds
AUTOR(ES)
Ching, Te May
RESUMO
An average of 540 picomoles of total adenosine phosphates was found in the embryo of mature seeds of ponderosa pine (Pinus ponderosa Laws.) and 1140 picomoles in the gametophyte. Adenylate energy charges were 0.44 and 0.26, respectively. After stratification, total adenosine phosphates increased 7-fold and 6-fold in embryo and gametophyte, respectively, and energy charges rose to 0.85 and 0.75. During germination, total adenosine phosphates increased to a 20-fold peak on the 9th day in gametophytic tissue, parallel with the peak of reserve regradation and organellar synthesis, and then decreased. In embryo and seedling, total adenosine phosphates elevated 80-fold with two distinct oscillating increases of AMP and ADP. The oscillating increases occurred before the emergence of radicle and cotyledons during which the highest mitotic index prevailed in all tissues. Energy charges fluctuated between 0.65 at the rapid cell dividing stage to 0.85 at the fully differentiated stage of the seedling, while energy charges remained around 0.75 in the gametophyte. These data indicated that the content of adenosine phosphates of germinating seeds reflects growth, organogenesis, and morphogenesis, and that a compartmentalized energy metabolism must exist in dividing and growing plant cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=366185Documentos Relacionados
- Glyoxysomes in Megagamethophyte of Germinating Ponderosa Pine Seeds 12
- Adenosine Phosphates in Germinating Radish (Raphanus sativus L.) Seeds 1
- Adenylate Energy Pool and Energy Charge in Maturing Rape Seeds 1
- Adenylate energy charge in Acholeplasma laidlawii.
- Adenylate energy charge in Saccharomyces cerevisiae during starvation.
