Contribuição ao estudo do crescimento de Candida utilis IZ-1840 em glicerol, adaptação de um modelo de inibição por substrato / Contribution to study of the growth of Candida utilis IZ-1840 in glycerol, adaptation by one model of substrate inhibition

AUTOR(ES)

Dante Augusto Moraes

DATA DE PUBLICAÇÃO

1994

RESUMO

The influence of initial substrate concentration in the growth of Candida utilis IZ-1840 in a buffered synthetic mediurn was studied utilizing glycerol (1,2,3-propanetriol) as an exc1usive carbon source, in the following concentrations: 15, 25, 35, 45, 55, 65 and 75 of in double assays. Characteristic kinetic behavior of substrate inhibition was detected, then one model equation of inhibition based on literature was adjusted for the maximum specific growth rate in function of the initial substrate concentration. From this equation it was possible to determine the value of the initial substrate concentration that do not occur growth inhibition, defined as S* (S* = 17,511 g/L).and the theoretical value of limit concentration where above this the growth inhibition becomes maximum which was defined as Sm (Sm = 175,4 g/L). The theoretical value for maximum specific growth rate, defmed as µ* corresponding to determined S* as initial substrate concentration was calculated in 0,225 1/h.

ASSUNTO(S)

candida utilis growth biotechnology crescimento substrate inhibition candida utilis biotecnologia inibição por substrato

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