Control of expression of the I factor, a LINE-like transposable element in Drosophila melanogaster.
AUTOR(ES)
Udomkit, A
RESUMO
I factors are LINE-like transposable elements in the genome of Drosophila melanogaster. They normally transpose infrequently but are activated in the germline of female progeny of crosses between males of a strain that contains complete elements, an I or inducer strain and females of a strain that does not, an R or reactive strain. This causes a phenomenon known as I-R hybrid dysgenesis. We have previously shown that the I factor promoter lies between nucleotides 1 and 30. Here we demonstrate that expression of this promoter is regulated by nucleotides 41-186 of the I factor. This sequence can act as an enhancer as it stimulates expression of the hsp7O promoter in ovaries in the absence of heat-shock. Within this region there is a site that is required for promoter activity and that is recognized by a sequence-specific binding protein. We propose that this protein contributes to the enhancer activity of nucleotides 41-186 and that reduced I factor expression in inducer strains is due to titration of this protein or others that interact with it.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=450260Documentos Relacionados
- Copy number control of a transposable element, the I factor, a LINE-like element in Drosophila
- BS a novel LINE-like element in Drosophila melanogaster.
- Identification of a potential RNA intermediate for transposition of the LINE-like element I factor in Drosophila melanogaster.
- High genetic instability of heterochromatin after transposition of the LINE-like I factor in Drosophila melanogaster
- Retrotransposition of a marked Drosophila line-like I element in cells in culture.