Conversion of barley SNPs into PCR-based markers using dCAPS method

AUTOR(ES)

Shahinnia, Fahimeh, Sayed-Tabatabaei, Badraldin Ebrahim

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

19/06/2009

RESUMO

Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.

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