Cosmid-derived map of E. coli strain BHB2600 in comparison to the map of strain W3110.
AUTOR(ES)
Birkenbihl, R P
RESUMO
A physical map for the genome of E. coli K12 strain BHB2600 was constructed by use of 570 cloned DNA elements (CDEs) withdrawn from a cosmid library. Dot blot hybridisation was applied to establish contig interrelations with subsequent fine mapping achieved by analysis of EcoR1 restriction patterns on Southern blots. The derived map covers nearly 95% of the E. coli genome resulting in 12 minor gaps. It may be compared to the almost complete map for strain W3110 of Kohara et al. (1). Except for one tiny gap (lpp,36.5') remaining gaps in BHB2600 do not coincide with those in W3110 so that both maps complement each other establishing an essentially complete clone represented map. Besides numerous minute differences (site and fragment gains and losses) both strains harbour at differing positions extended rearrangements flanked by mutually inverted repetitive elements, in our case insertion elements (IS1 and IS5).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=318094Documentos Relacionados
- A highly polymorphic locus in human DNA revealed by cosmid-derived probes.
- Variation in RNA polymerase sigma subunit composition within different stocks of Escherichia coli W3110.
- Determinants for overproduction of the Pseudomonas oleovorans cytoplasmic membrane protein alkane hydroxylase in alk+ Escherichia coli W3110.
- Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome.
- A copy of insertion element IS5 is present within tnaB in the Kohara library of Escherichia coli W3110.
