CR-EST: a resource for crop ESTs
AUTOR(ES)
Künne, C.
FONTE
Oxford University Press
RESUMO
The crop expressed sequence tag database, CR-EST (http://pgrc.ipk-gatersleben.de/cr-est/), is a publicly available online resource providing access to sequence, classification, clustering and annotation data of crop EST projects. CR-EST currently holds more than 200 000 sequences derived from 41 cDNA libraries of four species: barley, wheat, pea and potato. The barley section comprises approximately one-third of all publicly available ESTs. CR-EST deploys an automatic EST preparation pipeline that includes the identification of chimeric clones in order to transparently display the data quality. Sequences are clustered in species-specific projects to currently generate a non-redundant set of ∼22 600 consensus sequences and ∼17 200 singletons, which form the basis of the provided set of unigenes. A web application allows the user to compute BLAST alignments of query sequences against the CR-EST database, query data from Gene Ontology and metabolic pathway annotations and query sequence similarities from stored BLAST results. CR-EST also features interactive JAVA-based tools, allowing the visualization of open reading frames and the explorative analysis of Gene Ontology mappings applied to ESTs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=540073Documentos Relacionados
- NEMBASE: a resource for parasitic nematode ESTs
- A Populus EST resource for plant functional genomics
- A Large Database of Chicken Bursal ESTs as a Resource for the Analysis of Vertebrate Gene Function
- Trypanosomatid EST: a neglected information resource regarding flagellated protozoa?
- PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries
