Critical roles of spo0A and spo0H in vegetative alkaline phosphatase production in Bacillus subtilis.
AUTOR(ES)
Hulett, F M
RESUMO
Growth conditions established to optimize vegetative alkaline phosphatase production and stability in Bacillus subtilis were used to compare alkaline phosphatase synthesis and secretion in isogenic strains JH646 (spo0A12) and JH646MS (spo0A12 abrB15). A mutation in spo0A blocked vegetative alkaline phosphatase production, and a second mutation at the abrB locus resulted in hyperinduction of vegetative alkaline phosphatase. Phosphate regulation of vegetative alkaline phosphatase synthesis was unaffected in the double mutant. spo0H, on a multicopy plasmid, partially overcame the spo0A effect.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211360Documentos Relacionados
- Bacillus subtilis spo0H gene.
- Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis.
- Mutations in pts cause catabolite-resistant sporulation and altered regulation of spo0H in Bacillus subtilis.
- Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis.
- Evidence that spo0A mutations are recessive in spo0A-/spo0A+ merodiploid strains of Bacillus subtilis.