Crystallization screening test for the whole-cell project on Thermus thermophilus HB8

AUTOR(ES)

Iino, Hitoshi

FONTE

International Union of Crystallography

RESUMO

Three conventional robots were subjected to a crystallization screening test involving 18 proteins from T. thermophilus HB8 using the sitting- and hanging-drop vapour-diffusion and microbatch methods. The number of diffraction-quality crystals and the amount of time required to obtain visible crystals depended greatly on the robots used. The combined use of different robots, especially for protein samples exhibiting low crystallization success rates, significantly increased the chance of obtaining diffraction-quality crystals.

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