Cultures of shiverer mutant cerebellum injected with normal oligodendrocytes make both normal and shiverer myelin.

AUTOR(ES)

Billings-Gagliardi, S

RESUMO

Earlier reports suggested that injecting normal optic nerve into organotypic cerebellar cultures from three of the central nervous system hypomyelinated mutant mice resulted in striking local increases in myelination of mutant axons. It has been questioned whether this myelin was produced by the introduced normal glia because there was no "marker" by which the genotype of an individual myelin sheath could be rigorously determined. The present study shows that the myelin sheaths of the central nervous system hypomyelinated mutant shiverer (shi/shi) can be distinguished in vitro from genetically normal myelin by their immunocytochemical reactions and ultrastructure. When shi/shi cultures are injected with normal optic nerve, they produce two kinds of myelin as distinguished by these techniques: shi/shi myelin throughout and, in addition, ultrastructurally and immunocytochemically normal myelin near the optic nerve. These results suggest that the primary defect of the shi/shi mutation involves the oligodendrocyte and support the earlier conclusion that in all of the glial injection experiments to date the genotype of the oligodendrocyte determines the phenotype of the myelin produced.

Documentos Relacionados

• Two large glycosylated polypeptides found in myelinating oligodendrocytes but not in myelin.
• Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young.
• The structure of bovine brain myelin proteolipid and its organization in myelin.
• Membrane particles on fracture faces of frozen myelin.
• Porphyrin-heme biosynthesis in organotypic cultures of mouse dorsal root ganglia. Effects of heme and lead on porphyrin synthesis and peripheral myelin.