Cyclic AMP-Mediated Inhibition of Cell Growth Requires the Small G Protein Rap1
AUTOR(ES)
Schmitt, John M.
FONTE
American Society for Microbiology
RESUMO
In many normal and transformed cell types, the intracellular second messenger cyclic AMP (cAMP) blocks the effects of growth factors and serum on mitogenesis, proliferation, and cell cycle progression. cAMP exerts these growth-inhibitory effects via inhibition of the mitogen-activated protein (MAP) kinase cascade. Here, using Hek293 and NIH 3T3 cells, we show that cAMP's inhibition of the MAP kinase cascade is mediated by the small G protein Rap1. Activation of Rap1 by cAMP induces the association of Rap1 with Raf-1 and limits Ras-dependent activation of ERK. In NIH 3T3 cells, Rap1 is required not only for cAMP's inhibition of ERK activation but for inhibition of cell proliferation and mitogenesis as well.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=86997Documentos Relacionados
- Cell-specific cyclic AMP-mediated induction of the PDGF receptor.
- Cyclic AMP-Mediated Induction of the Cyclic AMP Phosphodiesterase of C-6 Glioma Cells
- Reversal of Cyclic AMP-Mediated Intestinal Secretion by Ethacrynic Acid
- Cyclic AMP-mediated induction of ornithine decarboxylase of glioma and neuroblastoma cells.
- Cyclic AMP-mediated regulation of excitation-contraction coupling in canine gastric smooth muscle.
