Cytochrome c in Hydrogenomonas eutropha1
AUTOR(ES)
Fang, Florence S.
RESUMO
A c-type cytochrome from Hydrogenomonas eutropha was purified 150-fold by butanol extraction, ammonium sulfate precipitation, and column chromatography. Three distinct c-type cytochromes were recovered which did not bind with either carbon monoxide or cyanide and hence did not appear to be denatured. Polyacrylamide gel electrophoresis indicated that one protein was acidic and the other two were basic. The acidic cytochrome c had a sedimentation coefficient of 3.46. Its amino acid composition was not markedly different from other bacterial cytochromes, but relative to mammalian cytochromes c it was low in lysine, threonine, and isoleucine and high in alanine and valine.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252297Documentos Relacionados
- Mixotrophic Growth of Hydrogenomonas eutropha
- NUTRITIONAL REQUIREMENTS FOR HYDROGENOMONAS EUTROPHA
- Mixotrophic Growth of Hydrogenomonas eutropha
- Factors Affecting the Synthesis and Degradation of Ribulose-1,5-Diphosphate Carboxylase in Hydrogenomonas facilis and Hydrogenomonas eutropha
- Nutritional Value of Lipids in Hydrogenomonas eutropha as Measured in the Rat1