Dark-field microscopy for detection of malaria in unstained blood films.

AUTOR(ES)

Jamjoom, G A

RESUMO

Pigment present in different forms of Plasmodium species infecting humans exhibits light scattering when blood films are viewed by dark-field microscopy. This facilitates the detection of parasite-infected cells owing to their brightly illuminated appearance. The technique was described long ago for the detection of certain highly pigmented forms of malarial parasites such as schizonts and gametocytes but has not found an application in routine diagnosis. Here, modifications are described which allow the detection of all forms of human malarial parasites, including ring forms, in unstained blood films. The technique offers the distinct advantages of rapid diagnosis, increased sensitivity, and adaptability to field work.

Documentos Relacionados

• Examination of bacterial flagellation by dark-field microscopy.
• Dark-Field Electron Microscopy of Thin Sections of Trichosporon cutaneum
• Dark-field Illumination for Photographing Precipitin Bands in Gel
• Simple Technique for Dark-Field Photography of Immunodiffusion Bands
• Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis.