Definition of Sequence Requirements for Latency-Associated Nuclear Antigen 1 Binding to Kaposi's Sarcoma-Associated Herpesvirus DNA
AUTOR(ES)
Srinivasan, Viswanathan
FONTE
American Society for Microbiology
RESUMO
In latent infection, Kaposi's sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen 1 (LANA1)-specific binding to KSHV terminal repeat DNA mediates multicopy episome persistence. We now use electrophoretic mobility shift assays to investigate LANA1 binding to its 20-bp cognate sequence. Mutations at positions 6, 7, and 8 (6CCC8) severely reduced LANA1 binding, whereas mutations at other positions only modestly reduced binding. Since 6CCC8 is in the 5′ half of an inverted repeat sequence, these results are consistent with an asymmetric role for the inverted repeat in LANA1 binding.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=533900Documentos Relacionados
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