Degradation of 2,4,6-trinitrotoluene by P. aeruginosa and characterization of some metabolites

AUTOR(ES)

Mercimek, Hatice Aysun, Dincer, Sadik, Guzeldag, Gulcihan, Ozsavli, Aysenur, Matyar, Fatih, Arkut, Afet, Kayis, Fikret, Ozdenefe, Melis Sumengen

FONTE

Braz. J. Microbiol.

DATA DE PUBLICAÇÃO

2015-03

RESUMO

Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitroexperiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites.

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