Deletion mapping of HLA and chromosome 6p genes.

AUTOR(ES)

Levine, F

RESUMO

We have analyzed a set of heterozygous mutants with deletions that encompass parts of HLA and surrounding regions of chromosome 6p. By a combination of Southern blotting, serologic, enzymatic, and cytogenetic analyses, we have ordered eight independent deletion break points into a sequence that divides chromosome 6p into six regions. The deletion mutants have been used in conjunction with the Southern blot technique to map HLA and other 6p gene probes into those regions. On the basis of these and other data we propose a genetic and physical map of HLA and surrounding regions of chromosome 6p. We find that for HLA probes, most of which hybridize with more than one gene, the multiple copies recognized by single probes map to single regions. Any chromosome 6p gene can now be regionally mapped by using these mutants.

Documentos Relacionados

• Duplication 6p and deletion 9p.
• Isolation and mapping of a polymorphic DNA sequence (pHHH171) on chromosome 6p [D6S38]
• Isolation and mapping of a polymorphic DNA sequence (pHHH157) on chromosome 6p [D6S29]
• Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting.
• Distal 6p deletion syndrome: a report of a case with anterior chamber eye anomaly and review of published reports.