Demonstration of Epstein-Barr virus in primary brain lymphoma by in situ DNA hybridisation in paraffin wax embedded tissue.
AUTOR(ES)
Murphy, J K
RESUMO
Tumour tissue from 29 patients with primary brain lymphoma was reviewed to determine if there was an aetiological association between Epstein-Barr virus and polyclonal and monoclonal lymphoproliferations. The morphology and immunophenotype in 24 patients for whom paraffin wax embedded tissue was available were studied. A high grade pleomorphic tumour morphology with plasmacytoid features was seen in 13 tumours. Because of the large number of pleomorphic lymphomas, all tumours were examined for the presence of the Epstein-Barr virus genome using in situ DNA hybridisation. A panel of three biotinylated probes to different sequences in the Epstein-Barr virus genome was used. Positive hybridisation with one or more probes was shown in tumours from 11 patients. The remaining tumours gave no hybridisation signal. There was no correlation between positive hybridisation and morphological subtype or clinical outcome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=502334Documentos Relacionados
- Detection of single copies of Epstein-Barr virus in paraffin wax sections by non-radioactive in situ hybridisation.
- Feasibility of in situ hybridisation with chromosome specific DNA probes on paraffin wax embedded tissue.
- In situ hybridisation for the identification of Helicobacter pylori in paraffin wax embedded tissue.
- Absence of Epstein-Barr virus RNA in multiple sclerosis as assessed by in situ hybridisation.
- DNA of Epstein-Barr Virus Detected in Tissue of Burkitt's Lymphoma and Nasopharyngeal Carcinoma