Dendritic cells, inducible nitric oxide synthase and citokines expression in pityriasis lichenoides skin lesions / Células dendríticas, expressão da forma induzida da óxido nítrico sintase e padrão de citocinas nas lesões de pitiríase liquenóide

AUTOR(ES)

Gabriella Di Giunta

DATA DE PUBLICAÇÃO

2008

RESUMO

BACKGROUND: Pityriasis lichenoides (PL) is a cutaneous disease of unknown etiology which has been regarded as an immunologically mediated reaction. Recently, it was reclassified in the group of Cutaneous Lymphoid T cell Dyscrasia. There are few reports addressing mainly T cell subsets in PL tissue reaction. METHODS: Skin biopsies taken from 34 patients with confirmed diagnosis of PL where classified as pityriasis lichenoides et varioloformis acuta (PLEVA) (n = 15) and pityriasis lichenoides chronica (PLC) (n = 19) according to histopathological features. The skin biopsies where subjected to immunohistochemical technique to demonstrate Langerhans cells, Factor XIIIa+ dermal dendrocytes, inducible nitric oxide synthase (iNOS) expression, tumor necrosis factor alfa (TNFalfa), interferon gama (IFNy) and interleukins (IL) 12 and 10. The ensuing results were compared among the two PL groups. The iNOS results in PL group were also compared to a normal skin control group (n = 10). RESULTS: In PLEVA lesions, there was a decrease in Langerhans cells population when compared to PLC lesions. The factor XIIIa+ dermal dendrocytes number did not differ among PL groups. There was a strong epidermal and dermal iNOS expression in both PL groups. A faint iNOS expression was observed in three specimens of the control group. A higher TNFalfa and INFy expression was observed in PLEVA lesions. The Langerhans cells decrease observed in those lesions was accompanied by higher TNFalfa and IL-10 expression. There was a significant correlation between factor XIIIa+ dermal dendrocytes population and dermal iNOS expression. CONCLUSIONS: PLEVA lesions displayed a decrease in Langerhans cells number, accompanied by higher TNFalfa and IL-10 expression. There was no difference in the amount of factor XIIIa+ dermal dendrocytes in PLEVA and PLC lesions. The study demonstrated, for the first time, the iNOS expression in PL lesions. The factor XIIIa+ dermal dendrocytes population correlated to dermal iNOS expression. TNFalfa and INFy expression correlated to inflammatory alterations observed in PLEVA lesions. There was a negative correlation between IL-12 and IL-10 expression in PL lesions. Dendritic cells participate in the PL spectrum of tissue reaction which is characterized by predominant TH1 cytokines milieu that favors iNOS expression.

ASSUNTO(S)

Óxido nítrico pityriasis lichenoides/physiopathology imunoistoquímica immunohistochemistry interleucinas/análise pitiríase liquenóide/fisiopatologia nitric oxide interleukins/analysis

Documentos Relacionados

• Células dendríticas plasmocitoides, dendrócitos dérmicos fator XIIIa positivos, macrófagos e expressão da forma induzida da óxido nítrico sintase na resposta tecidual cutânea de leishmaniose tegumentar americana
• Activity of the nitric oxide synthase/nitric oxide system in bovine oocytes
• Estudo da associação de polimorfismos genéticos da sintase induzida do óxido nítrico (inos) com migrânea = : Inducible nitric oxide synthase haplotype associated with migraine and aura
• Efeito da óxido nítrico sintase na dor neuropática.
• Expression of Inducible Nitric Oxide Synthase in Skin Lesions of Patients with American Cutaneous Leishmaniasis