Densidade e área do perfil celular dos neurônios mioentéricos reativos a acetilcolinesterase, NADH e NADPH-diaforase positivos do ceco de ratos (Rattus norvergicus) adultos / Density and area of the cellular outline of the myoenteric plexus reactive to Acethylcholinesterase, NADH and NADPH diaphorase positives of the caecum of adult (Rattus norvergicus) rats

AUTOR(ES)

Carlos Eduardo Seyfert

DATA DE PUBLICAÇÃO

2003

RESUMO

Beginning in the terminal ileum where the ileal papilla opens, the caecum is the initial portion of the large intestine, where part of the digestion of the cellulose coming from the food and the absorption of its products take place. This study had the purpose of evaluating the density and the cell profile area of the myoenteric neurons in different regions of the caecum of adult rats aging seven months (384 g body weight), using the techniques of acetylcholinesterase (AChE), NADH- and NADPH-diaphorase. For that, the caecum was divided into four regions: next to the caecal ampulla (PA), apical mesenteric (AM), basal antimesenteric (BA) and apical antimesenteric (AA). It was observed that, in what concerns the neuronal density, there is a significant difference for the regions studied, the PA region exhibiting the greatest amount of neurons with all the techniques employed. On average, in the 80 microscopic fields (17.92 m2) counted, 1,479 ± 357 neurons were found with AChE, 717 ± 142 with NADH-d and 240 ± 26 with NADPH-d. As for the cell profile of the neurons, the Variance Analysis showed that there is not a significant difference among the regions. The smallest and the largest cellular profiles were observed at the BA region with the techniques of AChE and NADPH-d, respectively, the smallest having 77.94 µm2 and the largest 1,579.64 µm2. According to the area of the cell profile, the neurons were grouped in three classes: small, medium and large. The most frequent class in all the regions studied and techniques employed was that of medium neurons, reaching 72.4% with AChE at the AA region, 68% in the PA region with NADH-d and 77.6% with NADPH-d in the PA region. Therefore, it was verified that there is a regional difference in the distribution of the neurons in the wall of the rat caecum.

ASSUNTO(S)

neurônio mioentérico plexo mioentério rat ceco ratos caecum myoenteric neuron myoenteric plexus

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