Deoxyribonucleic Acid-Envelope Complexes Isolated from Escherichia coli by Free-Flow Electrophoresis: Biochemical and Electron Microscope Characterization
AUTOR(ES)
Olsen, William L.
RESUMO
A procedure for the preparative isolation of Escherichia coli cell wall, membrane, and deoxyribonucleic acid (DNA)-envelope complex fragments has been developed. The envelope fragments were produced by controlled mechanical cell breakage and isolated by density gradient centrifugation and subsequent preparative free-flow electrophoresis. The DNA-envelope complex fragments were shown to contain biochemical markers of both the cell wall and the membrane and by electron microscopy to be cell envelope fragments containing wall/membrane adhesion zones.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246800Documentos Relacionados
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