Deoxyribonucleic Acid Heterogeneity Between Human and Murine Strains of Chlamydia trachomatis1

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We compared the polynucleotide sequence relationships of three strains of Chlamydia trachomatis of human origin (MRC-1/G, TW-3, and Lgv), one of murine origin (MoPn), and the MN strain of C. psittaci. The four strains of C. trachomatis have the same base ratio, about 42.5 moles per cent guanine plus cytosine, which is significantly higher than the base ratio of MN (39.5). Single strands of deoxyribonucleic acid (DNA) fragments of MRC-1/G reassociated with immobilized DNA of TW-3 and Lgv almost as well as with the homologous DNA. The duplexes produced in these reactions were about equally thermostable. On the other hand, reassociations between MRC-1/G and MoPn involved 60 or 30% of the DNA, depending on the stringency of the conditions for reassociation, and the duplexes were thermolabile. MoPn reassociated only to a very small degree with MN. We also compared glucose catabolism of MRC-1/G, MoPn, and MN under several sets of conditions. These tests failed to reveal any qualitative phenotypic differences among the three strains. It can be concluded that, judging by polynucleotide sequence, the three human strains of C. trachomatis are closely related but appreciably different from a murine strain.

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