Dephosphorylation of histones H1 and H3 during the isolation of metaphase chromosomes.

AUTOR(ES)

D'Anna, J A

RESUMO

Histones have been extracted from isolated metaphase chromosomes prepared by the method of Wray and Sutbblefield [Exp. Cell Res 59, 469-478 (1970)] and by a Nonidet P-40 detergent procedure based on the method of Wigler and Axel [Nucleic Acids Res. 3, 1463-1471 (1976)]. Analysis of the densitometer profiles of long polyacrylamide gels shows that the mitotic phosphorylations of histone H1 (H1M) and histone H3 are extensively depleted during chromosome isolation. These data indicate that CHO metaphase chromosomes prepared by standard methodologies do not represent in vivo chromosomes with respect to their histone phosphorylations; therefore, current chemical and structural studies of isolated metaphase chromosomes may require further clarification.

Documentos Relacionados

• Isolation and characterization of human metaphase chromosomes.
• Effect of thyrotropin on 32P-labelled histones H1 and H3 in specific populations of nucleosomes in the thyroid.
• INCORPORATION OF H3 CYTIDINE AND H3 THYMIDINE INTO GIANT CHROMOSOMES OF DROSOPHILA DURING PUFF FORMATION
• Nucleosomes in metaphase chromosomes.
• Histones H3 and H4 interact with the ends of nucleosome DNA.