Deposition of histones onto replicating chromosomes.

AUTOR(ES)

Jackson, V

RESUMO

The mode of distribution of newly synthesized and pre-existing histones has been studied during the process of chromosome replication. Newly synthesized histone was labeled with [3H]lysine and newly synthesized DNA was density labeled with iododeoxyuridine. The histone was covalently linked to DNA, and radiolabeled histone was analyzed on CsCl density gradients. We have defined conditions that do not give rise to histone randomization during isolation, and also developed a method of defining the distribution of histones in chromatin on a density gradient in the unavoidable presence of nonhistone protein. Three possible modes of distribution of histone onto the replicating chromosome can be conceived; we describe experiments designed to distinguish unequivocally among these possibilities and conclude that histones are deposited randomly onto the chromosome.

Documentos Relacionados

• DNA synthesis by partially purified replicating simian virus 40 chromosomes.
• Lack of positional requirements for autonomously replicating sequence elements on artificial yeast chromosomes.
• Dephosphorylation of histones H1 and H3 during the isolation of metaphase chromosomes.
• Complementation of the beige mutation in cultured cells by episomally replicating murine yeast artificial chromosomes.
• Monoclonal antibody that preferentially binds polylysine, polyarginine, and histones and selectively decorates nuclei and chromosomes.