Desenvolvimento e validação de metodos analititicos para a deteminação de residuos de estreptomicina e diidroesteptominicina em leite bovino (ELISA, LC-APCI-MS/MS QToF) / Development and validation of analytical methos for the determination of streptomycin and ddihydrostreptomycin residues in milk (ELISA, LC-APCI-MS/MS QToF)

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

31/07/2009

RESUMO

Veterinary drugs pose a risk to human health when there residual levels in foods exceed the maximum residue limit (MRL). Knowledge of the extent of human exposure to these compounds is necessary to provide consumer protection. To achieve this, it is essential that validated analytical methods are available to ensure reliable results. The aim of the present work was to develop and validate analytical methods for the analysis of streptomycin and dihydrostreptomycin residues in milk, and to employ these methods for analysis of samples marketed in the region of Campinas, São Paulo. The developed and/or validated methods include enzyme immunoassay (ELISA) and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS QToF). Method validation was performed according to the recommendations established by European Community and IUPAC. The following parameters were evaluated: analytical curve, linearity, sensitivity, precision (intra and inter-day repeatability), accuracy and the detection and quantification limits. The results demonstrated the effectiveness of ELISA and LCMS/MS methods for trial and confirmatory analyses, respectively, of streptomycin and dihydrostreptomycin in milk at concentrations below the established MRL (200µg kg-1). In the analyzed samples streptomycin and dihydrostreptomycin were not found at detectable levels

ASSUNTO(S)

contaminação medicamentos veterinarios estreptomicina diidroestretomicina contamination veterinarian drug residues streptomycin dihydroesptretomicyn

ACESSO AO ARTIGO

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