Destabilization of nucleosomes by an unusual DNA conformation adopted by poly(dA)⋅poly(dT) tracts in vivo
AUTOR(ES)
Shimizu, Mitsuhiro
FONTE
Oxford University Press
RESUMO
Poly(dA)⋅poly(dT) tracts are common and often found upstream of genes in eukaryotes. It has been suggested that poly(dA)⋅poly(dT) promotes transcription in vivo by affecting nucleosome formation. On the other hand, in vitro studies show that poly(dA)⋅poly(dT) can be easily incorporated into nucleosomes. Therefore, the roles of these tracts in nucleosome organization in vivo remain to be established. We have developed an assay system that can evaluate nucleosome formation in yeast cells, and demonstrated that relatively longer tracts such as A15TATA16 and A34 disrupt an array of positioned nucleosomes, whereas a shorter A5TATA4 tract is incorporated in positioned nucleosomes of yeast minichromosomes. Thus, nucleosomes are destabilized by poly(dA)⋅poly(dT) in vivo in a length-dependent manner. Furthermore, in vivo UV footprinting revealed that the longer tracts adopt an unusual DNA structure in yeast cells that corresponds to the B′ conformation described in vitro. Our results support a mechanism in which a unique poly(dA)⋅ poly(dT) conformation presets chromatin structure to which transcription factors are accessible.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=313933Documentos Relacionados
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