Detection and Enumeration of Vibrio vulnificus in Oysters from Two Estuaries along the Southwest Coast of India, Using Molecular Methods
AUTOR(ES)
Parvathi, Ammini
FONTE
American Society for Microbiology
RESUMO
This study was conducted to understand the seasonal distribution of Vibrio vulnificus in oysters from two estuaries and the effect of environmental factors on the abundance of V. vulnificus in tropical waters. V. vulnificus was detected in 56.6% of the samples tested by colony hybridization with an alkaline phosphatase-labeled oligonucleotide probe (VV-AP), and the counts ranged from <10/g during the summer months to 103/g in the monsoon season at both sites. The density of V. vulnificus appeared to be controlled more by salinity than by temperature. A nested PCR used in this study detected V. vulnificus in 85% of the samples following 18 h of enrichment in alkaline peptone water.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=525118Documentos Relacionados
- Uptake and clearance of Vibrio vulnificus from Gulf coast oysters (Crassostrea virginica).
- Isolation and Characterization of Vibrio vulnificus from Two Florida Estuaries
- Evaluation of Two Direct Plating Methods Using Nonradioactive Probes for Enumeration of Vibrio parahaemolyticus in Oysters
- Virulent strains of Vibrio vulnificus isolated from estuaries of the United States West Coast.
- Effect of time and temperature on multiplication of Vibrio vulnificus in postharvest Gulf Coast shellstock oysters.