Detection and Localization of Virus-Specific DNA by In Situ Hybridization of Cells During Infection and Rapid Transformation by the Murine Sarcoma-Leukemia Virus
AUTOR(ES)
Loni, Maria Carla
RESUMO
Cytological preparations of interphase nuclei and chromosomes from mouse 3T6 cells prepared at various times after infection with the murine sarcomaleukemia virus complex were hybridized with the [3H]DNA product of the viral RNA-directed DNA polymerase. While uninfected nuclei had an average of 4 autoradiographic grains, infected nuclei had 30 grains at 5 hr after infection and 63-65 grains at 11 and 25 hr. Virus-specific grains were localized in the chromocenters of interphase nuclei and were found also in the centromeric heterochromatin region of metaphase chromosomes. These findings provide evidence that the viral RNA-directed DNA polymerase functions to synthesize virus-specific DNA early after infection and that newly synthesized viral DNA rapidly becomes associated with or integrated into specific intranuclear sites.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433784Documentos Relacionados
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