Detection and molecular characterization of gene 3 and 5 of turkey coronavirus (TCoV) from turkeys with severe enteritis in Brazil. / Detecção e caracterização molecular do gene 3 e 5 do coronavírus de perus (TCOV) isolados de perus com severa enterite no Brasil.
AUTOR(ES)
Amarilis Novaes D Elboux Bünger
DATA DE PUBLICAÇÃO
2009
RESUMO
Turkey coronavirus (TCoV) is causative agent associated to Poult Enteritis and Mortality Syndrome (PEMS) in turkeys wideworld. The disease is characterized by an acute highly contagious enteric disease of turkeys characterized by depression, anorexia, diarrhea and high mortality in co mMercial turkey flocks. The presence of turkey coronavirus (TCoV) in 29 intestinal content samples from turkey flocks aged between 10 and 104 days with severe enteritis was monitored in the period of 2004 to 2006. TCoV detection was accomplished by the reverse transcriptase-polymerase chain reaction (RT-PCR), through amplification of the 3´UTR region, followed by amplification of genes 3 and 5. Molecular characterization of the viruses was done through amplification of genes 3 and 5, and showed evidence of genetic similarity between them, although they differed of sequences of other TCoVs described in the literature. In relation to gene 3, samples showed greater relationship with chicken infectious bronchitis virus (IBV), and while gene 5 showed greater identity with pheasant coronavirus (PhCoV). Our results suggest that the strategy of amplification of the 3´UTR region has proved to an effective means of detection of TCoV in intestinal contents.
ASSUNTO(S)
análise filogenética disease genética molecular microbiology doenças turkeys coronavirus gene 5 microbiologia rt-pcr enterite animal rt-pcr gene 3 phylogenetic analysis gene 5 molecular characterization animal enteritis molecular genetics caracterização molecular coraonavírus de perus gene 3
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