Detection and Quantification of Pfiesteria piscicida by Using the Mitochondrial Cytochrome b Gene†

AUTOR(ES)

Zhang, Huan

FONTE

American Society for Microbiology

RESUMO

Mitochondrial cytochrome b was isolated from the dinoflagellate Pfiesteria piscicida, and the utility of the gene for species identification was examined. One of the primer sets designed was shown to be highly specific for P. piscicida. A time step PCR protocol was used to demonstrate the potential of this primer set for quantification of this species.

Documentos Relacionados

• Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates
• Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates†
• Characterization of the rRNA Locus of Pfiesteria piscicida and Development of Standard and Quantitative PCR-Based Detection Assays Targeted to the Nontranscribed Spacer
• Characterization of Ichthyocidal Activity of Pfiesteria piscicida: Dependence on the Dinospore Cell Density
• Mitochondrial Cytochrome b Gene Analysis of Aspergillus fumigatus and Related Species