Detection of a single base exchange in PCR-amplified DNA fragments using agarose gel electrophoresis containing bisbenzimide-PEG.

AUTOR(ES)

Müller, M

RESUMO

Using PCR fragments of known sequences derived from isolates of two related fungal species, simple submarine electrophoresis in agarose gels containing a bisbenzimide-PEG conjugate (H.A.-Yellow) has been shown to be capable of distinguishing DNA fragments 567 bp long which differ by as little as a single base change. However, only changes affecting bisbenzimide binding sites (which consist of at least four consecutive A/T bases) alter mobility; other changes are ineffective. A second ligand (H.A.-Red) with high G/C specificity is suggested which may be as effective in detecting other sequence changes.

Documentos Relacionados

• A simple and rapid electrophoresis method to detect sequence variation in PCR-amplified DNA fragments.
• Branch migration inhibition in PCR-amplified DNA: homogeneous mutation detection
• Rapid generation of specific single-stranded template DNA from PCR-amplified material.
• Identification of bacteria in a biodegraded wall painting by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
• Direct sequencing of PCR-amplified junction fragments from tandemly repeated transgenes.