Detection of Bacteria Carrying the stx2 Gene by In Situ Loop-Mediated Isothermal Amplification
AUTOR(ES)
Maruyama, Fumito
FONTE
American Society for Microbiology
RESUMO
A new in situ DNA amplification technique for microscopic detection of bacteria carrying a specific gene is described. Loop-mediated isothermal amplification (LAMP) was used to detect stxA2 in Escherichia coli O157:H7 cells. The mild permeabilization conditions and low isothermal temperature used in the in situ LAMP method caused less cell damage than in situ PCR. It allowed use of fluorescent antibody labeling in the bacterial mixture after the DNA amplification for identification of E. coli O157:H7 cells with an stxA2 gene. Higher-contrast images were obtained with this method than with in situ PCR.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=169117Documentos Relacionados
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