Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo
AUTOR(ES)
Nascimento, Elvira Maria Mendes, Gehrke, Flávia de Sousa, Maldonado, Rosa Amélia, Colombo, Silvia, Silva, Luiz Jacintho da, Schumaker, Teresinha Tizu Sato
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2005-05
RESUMO
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
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