Detection of group B streptococcal antigen in body fluids by a latex-coupled monoclonal antibody assay.
AUTOR(ES)
Rench, M A
RESUMO
A commercially available latex agglutination reagent, Directigen (Hynson, Westcott & Dunning, Div. Becton Dickinson & Co., Baltimore, Md.), in which a murine monoclonal antibody to group B streptococcal (GBS) antigen is the active component, was evaluated by using body fluid specimens from 94 sick infants. Antigen was detected in one or more admission specimens from 18 of 19 (94.7%) infants with symptomatic GBS infection. In 15 patients with GBS meningitis, cerebrospinal fluid, serum, and 10-fold-concentrated urine specimens were positive in 87, 50, and 100%, respectively. GBS antigen was detected in 50% of unconcentrated urines, but in no sera from infants with nonmeningitic GBS sepsis. Among specimens from 27 infants with invasive infection due to organisms other than GBS and from 48 culture-negative sick infants, false-positive latex agglutination reactions occurred in only 4 (9.5%) urine specimens and in no cerebrospinal fluid or serum specimens. The 94.7% sensitivity and specificity of the Directigen GBS test indicate that it is a useful reagent for the rapid diagnosis of invasive GBS infection in young infants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=271457Documentos Relacionados
- Rapid detection of group B streptococcal antigen by monoclonal antibody sandwich enzyme assay.
- Detection of group B streptococcal antigen in necropsy specimens using monoclonal antibody and immunoperoxidase staining.
- Assay of antibody to group A streptococcal carbohydrate by enzyme-linked immunosorbent assay.
- Monoclonal Antibody to Streptococcal Group B Carbohydrate: Applications in Latex Agglutination and Immunoprecipitin Assays
- Identification of Haemophilus influenzae type b by a monoclonal antibody coagglutination assay.