Detection of hepatitis A virus by extraction of viral RNA and molecular hybridization.
AUTOR(ES)
Ticehurst, J R
RESUMO
Hepatitis A virus (HAV) RNA was extracted from cell culture, serum, liver, and feces and then detected by molecular hybridization with cloned HAV cDNA. Hybridization was approximately 10-fold more sensitive than immune electron microscopy or radioimmunoassay was and less sensitive than was assays of HAV infectivity in primates or in cell culture. As little as 10(3) 50% infective doses of HAV, or approximately 0.1 pg of viral RNA, was detected by this method. Analysis of fecal specimens from an experimentally infected marmoset and an epidemic of hepatitis A showed that HAV excretion could often be detected later in the illness by hybridization than by radioimmunoassay. This technique should be widely applicable for detection and analysis of HAV RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269349Documentos Relacionados
- Detection of dengue-2 viral RNA by reversible target capture hybridization.
- Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR
- Detection of respiratory syncytial virus in nasopharyngeal secretions by DNA-RNA hybridization.
- Detection of labelled RNA species by contact hybridization.
- Cytomegalovirus in urine: detection of viral DNA by sandwich hybridization.