Detection of intracytoplasmic cytokines by flow cytometry

AUTOR(ES)

Santiago, Marta A, Luca, Paula M De, Bertho, Álvaro L, Azeredo-Coutinho, Rilza BG, Coutinho, Sérgio G

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2000-06

RESUMO

Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.

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