Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus
AUTOR(ES)
Custodio, Luiz Antonio, Saito, Alexandre, Amarante, Marla Karine, Fujita, Thiago Cezar, Perim, Aparecida de Lourdes, Costa, Ivete Conchon, Felipe, Ionice, Jankevicius, Shiduca Itow
FONTE
Brazilian Archives of Biology and Technology
DATA DE PUBLICAÇÃO
2012-06
RESUMO
In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.
Documentos Relacionados
- Polymerase chain reaction for detection of Mycobacterium leprae in nasal swab specimens.
- Sera of leprosy patients with type 2 reactions recognize selective sequences in Mycobacterium leprae recombinant LSR protein.
- Detection of Mycobacterium leprae nasal carriers in populations for which leprosy is endemic.
- Critical residues of the Mycobacterium leprae LSR recombinant protein discriminate clinical activity in erythema nodosum leprosum reactions.
- Detection of Mycobacterium leprae DNA by polymerase chain reaction in the blood and nasal secretion of Brazilian household contacts
