Detection of naturally occurring enteroviruses in waters by reverse transcription, polymerase chain reaction, and hybridization.
AUTOR(ES)
Kopecka, H
RESUMO
Comparison in virus-seeded mineral water of three detection methods for enteroviruses, direct hybridization, cell culture, and reverse transcription into cDNA followed by polymerase chain reaction and hybridization, showed that the last procedure was 10 to 1,000 times more sensitive than detection by cell culture and 10(5) to 10(7) times more sensitive than direct hybridization. The presence of naturally occurring enteroviruses was also demonstrated in activated sludge and in concentrated and non-concentrated surface water samples by reverse transcription-polymerase chain reaction-hybridization. However, in activated sludge and in concentrated surface waters, enzymatic amplification was sometimes inhibited by contaminants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202263Documentos Relacionados
- Comparison of reverse transcription polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine epidemic diarrhea virus in pigs
- Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization.
- Detection of enteroviruses by spot hybridization.
- Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization.
- Detection of Helicobacter pylori in dental plaque by reverse transcription-polymerase chain reaction.
