Detection of the surface-exposed 18-kilodalton binding protein in Chlamydia trachomatis by immunogold staining.
AUTOR(ES)
Gray, G J
RESUMO
Dot-blot analysis of Chlamydia trachomatis elementary bodies (EBs) with monospecific polyclonal antibodies demonstrated that the 18-kilodalton binding protein is surface exposed. Immunoelectron microscopy with whole serovar L2 EBs and ultrathin sections confirmed this finding. In addition, only the extracellular EBs and not the intracellular reticulate bodies were labeled with immunogold.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=209173Documentos Relacionados
- Effect of proteolytic cleavage of surface-exposed proteins on infectivity of Chlamydia trachomatis.
- Surface-exposed protein antigens of the gonococcal outer membrane.
- Ultrastructural study of Chlamydia trachomatis surface antigens by immunogold staining with monoclonal antibodies.
- Characterization of kinetics and target proteins for binding of human complement component C3 to the surface-exposed outer membrane of Chlamydia trachomatis serovar L2.
- Conformational Dependence of Anaplasma marginale Major Surface Protein 5 Surface-Exposed B-Cell Epitopes†