Detection of Trypanosoma cruzi DNA within murine cardiac tissue sections by in situ polymerase chain reaction
AUTOR(ES)
Lane, Joshua E, Ribeiro-Rodrigues, Rodrigo, Olivares-Villagómez, Danyvid, Vnencak-Jones, Cindy L, McCurley, Thomas L, Carter, Clint E
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2003-04
RESUMO
The use of in situ techniques to detect DNA and RNA sequences has proven to be an invaluable technique with paraffin-embedded tissue. Advances in non-radioactive detection systems have further made these procedures shorter and safer. We report the detection of Trypanosoma cruzi, the causative agent of Chagas disease, via indirect and direct in situ polymerace chain reaction within paraffin-embedded murine cardiac tissue sections. The presence of three T. cruzi specific DNA sequences were evaluated: a 122 base pair (bp) sequence localized within the minicircle network, a 188 bp satellite nuclear repetitive sequence and a 177 bp sequence that codes for a flagellar protein. In situ hybridization alone was sensitive enough to detect all three T. cruzi specific DNA sequences.
Documentos Relacionados
- Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction.
- Polymerase chain reaction-based detection of Trypanosoma cruzi DNA in serum.
- A simplified method for sample collection and DNA isolation for polymerase chain reaction detection of Trypanosoma rangeli and Trypanosoma cruzi in triatomine vectors
- Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus
- Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis.