Detection range verification of urinary ethyl glucuronide between chronic ethanol users and social drinkers by gas chromatography/mass spectrometry analysis / "Verificação da janela de detcção do etilglicuronídeo urinário entre usuários crônicos e bebedores sociais de etanol por cromatografia em fase gasosa acoplada à espectrometria de massas"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The detection range of the ethylglicuronide (EtG) seems to vary in accordance to the intensity of ethanol consumption. Thus, the objective of the present work was to develop and validate a method using extraction in solid phase and GCMS for the determination of urinary EtG, in order to verify its detection window in a group of social drinkers and chronic ethanol users. The limits of detection and quantification were 0,2 and 0.1 mg/L, respectively. The method showed to be linear in the interval of 0,2 to 100,0 mg/L (r2>0,99), and presented good accuracy and precision. The urinary EtG detection range in samples of five volunteers after controlled ingestion of 0,5 g/kg of ethanol varied between 24 to 35 hours. Urine samples were also collected from 14 patients from a recovery clinic. The results showed that the majority of them probably consumed ethanol, which compromised the interpretation of the results. In the cases where the urinary concentration of EtG decayed with time, the detection window varied from 72 to 120 hours. The method showed to be useful to monitor the ethanol abstinence during treatment of alcoholic patients or the consumption of only one ethanol low dose.

ASSUNTO(S)

etilglicuronídeo gas chromatography/mass spectrometry cromatografia em fase gasosa acoplada à espectrometria de massas extração em fase sólida biomarker for ethanol consumption marcador de consumo de etanol ethylglucuronide ethanol solid phase extraction etanol

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