Determination of eye irritation potential of low-irritant products: comparison of in vitro results with the in vivo draize rabbit test

AUTOR(ES)

Nóbrega, Andréa Martins da, Alves, Eloisa Nunes, Presgrave, Rosaura de Farias, Costa, Rodrigo Netto, Delgado, Isabella Fernandes

FONTE

Brazilian Archives of Biology and Technology

DATA DE PUBLICAÇÃO

2012-06

RESUMO

In an attempt to build the evaluation strategies to assess the human eye irritation, a reassessment of some in vitro tests is necessary, particularly concerning the non-irritants, mild and moderate irritants. Thus, the correlations between results obtained from the Draize test with the in vitro methods HET-CAM (Hens Egg Test-Chorion Allantonic Membrane) and RBC haemolysis assay to assess the ocular irritancy potential of 20 eye drops were examined. Parameters such as accuracy (%) and specificity (%) were determined. All results were correlated with RBC correctly with the results obtained in the Draize test. The HET-CAM presented four false-positive results, showing a tendency of data overestimation. Despite the high specificity provided mainly by the RBC, it would be necessary to test a wider range of products representing all the scales of irritation to confirm its ability to be used as a first alternative to test products that could be presumptive non-irritants.

Documentos Relacionados

• A Reassessment of the in vitro total protein content determination (TPC) with SIRC and 3T3 cells for the evaluation of the ocular irritation potential of shampoos: comparison with the in vivo Draize rabbit test
• In vitro citotoxicity test as an alternative to in vivo Draize test for the evaluation of cosmetic
• CINEMATOGRAPH EYE IRRITATION
• Evaluation of the In Vitro Pyrogen Test System Based on Proinflammatory Cytokine Release from Human Monocytes: Comparison with a Human Whole Blood Culture Test System and with the Rabbit Pyrogen Test
• Marine natural products: chemical and biological potential of seaweeds and their endophytic fungi